Triton X-100 Lysis Buffer 2X, 7.4 pH, 250mL
genuine image
Triton X-100 Lysis Buffer 2X, 7.4 pH, 250mL
Manufactured in an ISO 9001 and 13485 certified facility in the USA, our buffers are vital for protein research and molecular biology.
Triton X-100 Lysis Buffer 2X, 7.4 pH, 250mL
Artigo n.º: 145283403

Triton X-100 Lysis Buffer 2X, 7.4 pH, 250mL

Artigo n.º: 145283403

MZN 8587

Price Details

Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )

*All items will import from EUA

Em estoque
EUA Importado da loja USA

QTY:

Encomende já e receba por volta de Sexta-Feira, Julho 10
Secured transactionsecured transaction

Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.

Os nossos principais Parceiros Logísticos
  • fedex
  • dhl
Manufactured in an ISO 9001 and 13485 certified facility in the USA, our buffers are vital for protein research and molecular biology.
Garantia U-Care:
Nenhuma
Selecione um plano
fast shipping

Fast
Shipping

free return

Devolução
gratuita*

Embalagem Segura

Embalagem Segura

Produtos 100% Originais

Produtos 100% Originais

pci-dss

Certificação PCI DSS

iso certified

Certificação ISO 27001

paypal payment
visa payment
mastercard payment
Note: Step Down Voltage Transformer required for using electronics products of EUA store (110-120). Recommended power converters Compre Agora.

O que se Destaca

Optimal pH Level
Maintains a stable pH of 7.4, ensuring effective lysis and preserving sample integrity for reliable results in various biological assays.
Concentrated Formula
This 2x lysis buffer allows for flexible use, reducing the volume needed for experiments while maintaining potent lysis capabilities, saving both time and resources.
Large Volume
The 250ml size offers extensive use for multiple experiments, making it a cost-effective solution for laboratories requiring consistent and high-quality sample preparation.

Detalhes do produto

Shop Triton X-100 Lysis Buffer 2X, 7.4 pH, 250mL online at a best price in Mozambique. B09V5PY8T5
Item Weight0.55 lbs (250 grams)

Quem Deverá Comprar?

Suitable For
  • Biochemists

    Ideal for biochemists conducting protein extraction and analysis in a laboratory setting with controlled conditions.

  • Cell Biologists

    Beneficial for cell biologists needing to lyse cells for studying cellular components and functions effectively.

  • Research Laboratories

    Suitable for various research laboratories requiring reliable lysis buffers to ensure reproducibility in experiments.

Not Suitable For
  • Non-technical Users

    Not suitable for individuals without technical skills who may not know how to properly use lysis buffers.

  • Inexperienced Researchers

    May not be ideal for inexperienced researchers who lack knowledge of buffer preparation and cellular lysis techniques.

  • Home Use

    Not recommended for home use due to specialized applications that require lab environments and equipment.

DESCRIÇÃO DO PRODUTO

Triton X-100 Lysis Buffer 2X, 7.4 pH, 250mL

Tem alguma Dúvida? Converse connosco

Perguntas e Respostas dos Clientes

  • pergunta: What is Triton X-100 Lysis Buffer used for?

    Resposta: Triton X-100 Lysis Buffer is primarily used in biochemistry and molecular biology for cell lysis and extraction of proteins. The 2X concentration and neutral pH of 7.4 make it ideal for disrupting cellular membranes while maintaining protein stability. This buffer is particularly valuable in experiments involving protein assays, enzyme activity studies, or Western blotting. Researchers often use it when isolating proteins from cultured cells or tissues, ensuring that even the most delicate proteins remain intact during extraction.
  • pergunta: How should I store Triton X-100 Lysis Buffer?

    Resposta: Triton X-100 Lysis Buffer should be stored at 4°C for short-term use or at -20°C for long-term storage. It's crucial to keep the buffer away from direct light to prevent degradation of the components. Proper storage ensures that the buffer remains effective for your applications, maintaining its capability to lyse cells efficiently. For repeated use, aliquoting into smaller portions can help mitigate freeze-thaw cycles, which can affect buffer performance.
  • pergunta: Can Triton X-100 Lysis Buffer affect protein function?

    Resposta: Yes, Triton X-100 Lysis Buffer can affect protein function, as it is a non-ionic detergent that solubilizes biological membranes. While it is effective in extracting proteins, high concentrations may alter their native structures or functions. To mitigate potential impact, it's recommended to optimize the concentration based on the specific application and protein being studied. In scenarios where protein activity is crucial, researchers may experiment with lower Triton X-100 concentrations or alternative lysis methods.
  • pergunta: What applications can I use Triton X-100 Lysis Buffer for?

    Resposta: Triton X-100 Lysis Buffer is versatile and can be employed in various applications, including protein extraction, preparing samples for SDS-PAGE, and immunoprecipitation. Its ability to solubilize proteins while preserving their activity makes it suitable for studies in cell signaling, enzymatic assays, and antigen detection. For example, researchers often utilize this buffer when isolating cytosolic proteins from cultured cells for detailed biochemical analysis, highlighting its importance in scientific research.
  • pergunta: Is Triton X-100 Lysis Buffer suitable for all cell types?

    Resposta: Triton X-100 Lysis Buffer is effective on a wide range of cell types, including mammalian, yeast, and bacterial cells. However, some cell types may require modifications in buffer conditions or additional reagents due to differences in cell wall structures or membrane composition. It's advisable to assess the lysis efficiency through preliminary experiments, adjusting the buffer conditions if necessary. This ensures optimal extraction and yields for the specific cell line or organism being studied.
  • pergunta: What are the safety precautions for using Triton X-100 Lysis Buffer?

    Resposta: While Triton X-100 is not classified as highly toxic, standard laboratory safety procedures should still be followed. Wearing appropriate personal protective equipment, such as gloves and goggles, is essential to avoid skin and eye irritation. Additionally, working in a fume hood or well-ventilated area can help mitigate inhalation risks. Proper disposal methods must also be adhered to, as waste containing Triton X-100 should be treated as chemical waste and disposed of accordingly.
  • pergunta: Can I use Triton X-100 Lysis Buffer for DNA extraction?

    Resposta: Triton X-100 Lysis Buffer is primarily designed for protein extraction, but it can aid in the lysis step during DNA extraction protocols. The detergent disrupts cell membranes and helps release nucleic acids. However, it’s essential to use it in conjunction with other reagents that specifically help precipitate and purify DNA. Often, researchers combine it with substances such as phenol-chloroform or ethanol to achieve optimal DNA yield and purity.
  • pergunta: What is the pH level of Triton X-100 Lysis Buffer?

    Resposta: The Triton X-100 Lysis Buffer has a pH level of 7.4, which is close to physiological pH. This neutral pH is important in maintaining protein stability and activity during cell lysis and extraction processes. When working with sensitive proteins, adjusting pH to physiological levels helps to ensure that enzymatic activities and structural conformations are preserved. This buffer is particularly beneficial in various biological assays where maintaining similar conditions to natural environments is crucial.
  • pergunta: Where can I buy Triton X-100 Lysis Buffer (2X), 7.4 pH, 250mL?

    Resposta: You can buy Triton X-100 Lysis Buffer (2X), 7.4 pH, 250mL from Ubuy in Mozambique. Ubuy is known for its wide selection of scientific reagents and laboratory supplies, making it a reliable source for this essential research product. By choosing Ubuy, you can easily find high-quality lab reagents to support your scientific endeavors.
  • pergunta: What are the main ingredients in Triton X-100 Lysis Buffer?

    Resposta: The main ingredient in Triton X-100 Lysis Buffer is Triton X-100, a non-ionic surfactant, which effectively disrupts cell membranes to facilitate protein extraction. Additionally, the buffer typically contains salts to maintain ionic strength, and sometimes buffering agents to help stabilize the solution at pH 7.4. The formulation may vary slightly depending on specific research needs, but the focus remains on maintaining cell integrity for better protein yield. This composition makes it suitable for a range of biological experiments.

eConLabSupply Buffers Editorial Review

Nenhuma revisão editorial encontrada

Avaliações e Classificações dos Clientes

5.0
1 classificações de clientes
  • 5 Estrela
    100%
  • 4 Estrela
    0%
  • 3 Estrela
    0%
  • 2 Estrela
    0%
  • 1 Estrela
    0%

Avaliar este produto

Partilhe as suas ideias com outros clientes

Histórico de preços do produto

Informação importante

  • Limitações: para produtos expedidos internacionalmente, tenha em atenção que qualquer garantia do fabricante pode não ser válida; opções de serviço do fabricante podem não estar disponíveis; manuais de produtos, instruções e avisos de segurança podem não estar nas línguas do país de destino; os produtos (e materiais que o acompanham) pode não ter sido concebido em conformidade com as normas, especificações e requisitos de rotulagem do país de destino; e os produtos podem não estar em conformidade com a voltagem e outras normas elétricas do país de destino (requerendo assim o uso de um adaptador ou conversor caso seja apropriado). O destinatário é responsável por garantir que o produto pode ser legalmente importado para o país de destino. Quando encomenda à Ubuy ou aos seus afiliados, o destinatário é o importador de registo e deve estar em conformidade com todas as leis e regulamentos do país de destino.
  • Nem todos os produtos listados na Ubuy estão à venda, uma vez que a Ubuy é um motor de busca global. Os produtos estão sujeitos a leis de exportação/comércio.

MZN 8587

Encomende já e receba por volta de Sexta-Feira, Julho 10

QTY:

Secured transactionsecured transaction

Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.

Os nossos principais Parceiros Logísticos
  • fedex
  • dhl
Entrega internacional mais rápida

Recursos e benefícios

  • Comprehensive range of biological buffers for research and diagnostics.
  • Critical for protein research, molecular biology, and characterization.
  • Produced in an ISO certified facility ensuring high quality.
  • Manufactured under stringent Good Manufacturing Practices.
  • Each lot goes through rigorous quality control tests.
  • Triton X-100 effectively lyses cells for protein extraction.